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KMID : 0357319960310020175
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Journal of the Korean Society for Microbiology
1996 Volume.31 No. 2 p.175 ~ p.182
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The Study on Siderophore Biosynthetic System in Pseudomonas Sp. PY002
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Abstract
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In iron-limited environment, strain PY002 produced yellow-green fluorescent pigment. The absorption spetra of a culture supernatant showed two peaks at 280 nm and 400 nm. Yellow-green pigment production was enhanced at 30℃but repressed at
37℃and
41℃.
To identify siderophore-producing clone, siderophore biosynthesis defective mutant MP114, was complemented by using triparental conjugation method. From the complementation exprements, pCOM61 was able to exhibit Flu+/Sid+ phenotype. The
restriction
map
of insert DNA from the pCOM61 was constructed using the restriction enzymes such as EcoRⅠ, HindⅢand XhoⅠ. By subcloning, it was revealed that EcoRⅠ-EcoRⅠfragment (7 kb) of pCOM61 chould complement the MP114.
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KEYWORD
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